A laboratory technique for replicating a specific genetic sequence for analysis. It uses reverse transcriptase, an enzyme, to convert one piece of RNA into one piece of DNA. This piece of DNA is then amplified (in large quantities) by another enzyme called DNA polymerase. The amplified DNA copies help determine whether a gene produces a particular mRNA molecule. RT-PCR can be used to detect changes in a gene or chromosome, as well as the activation of specific genes, which can help with disease diagnosis, such as cancer. It can also be used to study the RNA of certain viruses, such as the human immunodeficiency virus (HIV) and the hepatitis C virus, to help with infection diagnosis and monitoring. The reverse transcription-polymerase chain reaction is another name for reverse transcription-polymerase chain reaction (RT-PCR).
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